Journal: Frontiers in Pharmacology
Article Title: Reversed role of CD36 deficiency in high-fat diet or methionine/choline-deficient diet-induced hepatic steatosis and steatohepatitis
doi: 10.3389/fphar.2025.1522177
Figure Lengend Snippet: CD36-deficiency reduces HFD-induced hepatic fatty acid synthesis, but aggravates MCD-induced oxidative stress. (A–C, E) Total RNA and proteins were extracted from a piece of liver. The proteins expression of nSREBP2, FASN, ACC1, CD36 (A) , PGC1α, CPT1α, PPARα, CD36 (Β) , SOD1 and SOD2 (E) were determined by Western blot. The mRNA expression of PGC1α and PPARα were determined qPCR (C) . (D) ROS in the liver was determined by Dihydrothidium (DHE) staining. *P < 0.05; ns, no significance (n = 6).
Article Snippet: Rabbit anti-acetyl-CoA carboxylase alpha (ACC1) polyclonal antibody was purchased from Cell Signaling Technology (Danvers, MA, United States).
Techniques: Expressing, Western Blot, Staining